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Hsc70 Recombinant antibody

Hsc70 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3H9

Cat no : 81719-2-RR

Synonyms

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Hsc70 Recombinant antibody (81719-2-RR)

    WB analysis using 81719-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81719-2-RR (Hsc70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Hsc70 Recombinant antibody (81719-2-RR)

    WB analysis of HeLa using 81719-2-RR

    WB result of Hsc70 antibody (81719-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HeLa cells.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81719-2-RR targets Hsc70 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Hsc70 fusion protein Ag1018
Full Name heat shock 70kDa protein 8
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007276
Gene Symbol HSC70
Gene ID (NCBI) 3312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

Protocols

Product Specific Protocols
WB protocol for Hsc70 antibody 81719-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols