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  • Featured Product
  • KD/KO Validated

FKBP10/FKBP65 Recombinant antibody

FKBP10/FKBP65 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

S94

Cat no : 81567-1-RR

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    WB analysis using 81567-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    WB analysis of HeLa using 81567-1-RR

    WB result of FKBP10/FKBP65 antibody (81567-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    IHC staining of human ovary tumor using 81567-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    IHC staining of human ovary tumor using 81567-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81567-1-RR

    Affinity and Kinetic Characterization of 81567-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81567-1-RR against Human FKBP10/FKBP65 were performed. The affinity constant is 32.4 pM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:11800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81567-1-RR targets FKBP10/FKBP65 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FKBP10/FKBP65 fusion protein Ag2814
Full Name FK506 binding protein 10, 65 kDa
Calculated Molecular Weight 582 aa, 64 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC016467
Gene Symbol FKBP10
Gene ID (NCBI) 60681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

Protocols

Product Specific Protocols
WB protocol for FKBP10/FKBP65 antibody 81567-1-RRDownload protocol
IHC protocol for FKBP10/FKBP65 antibody 81567-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols