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VDAC1/Porin Recombinant antibody

VDAC1/Porin Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F4

Cat no : 81538-1-RR

Synonyms

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Recombinant antibody (81538-1-RR)

    WB analysis using 81538-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81538-1-RR (VDAC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IHC staining of mouse liver using 81538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IHC staining of mouse liver using 81538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81538-1-RR (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using VDAC1/Porin Recombinant antibody (81538-1-RR)

    IF Staining of human liver using 81538-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VDAC1/Porin antibody (81538-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC1/Porin Recombinant antibody (81538-1-RR)

    FC experiment of HepG2 using 81538-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug VDAC1/Porin Recombinant antibody (81538-1-RR, Clone:2F4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inMDA-MB-231 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

81538-1-RR targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name voltage-dependent anion channel 1
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberNM_003374
Gene Symbol Porin
Gene ID (NCBI) 7416
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Protocols

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
IHC protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
IF protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
FC protocol for VDAC1/Porin antibody 81538-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Iron-frataxin involved in the protective effect of quercetin against alcohol-induced liver mitochondrial dysfunction

Authors - Jingjing Liu
View Article