• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

KGA/GAC Recombinant antibody

KGA/GAC Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4C20

Cat no : 81486-1-RR

Synonyms

GLS, KGA, GAM, GAC, EC:3.5.1.2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using GLS Recombinant antibody (81486-1-RR)

    WB analysis of A549 using 81486-1-RR

    WB result of GLS antibody (81486-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLS transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using GLS Recombinant antibody (81486-1-RR)

    WB analysis using 81486-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81486-1-RR (GLS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GLS Recombinant antibody (81486-1-RR)

    IHC staining of mouse brain using 81486-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81486-1-RR (GLS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GLS Recombinant antibody (81486-1-RR)

    IF Staining of A549 using 81486-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using GLS antibody (81486-1-RR, Clone: 4C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81486-1-RR

    Affinity and Kinetic Characterization of 81486-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81486-1-RR against Human GLS were performed. The affinity constant is 0.182 nM.

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81486-1-RR targets KGA/GAC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KGA/GAC fusion protein Ag3505
Full Name glutaminase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight58 kDa, 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Protocols

Product Specific Protocols
WB protocol for KGA/GAC antibody 81486-1-RRDownload protocol
IHC protocol for KGA/GAC antibody 81486-1-RRDownload protocol
IF protocol for KGA/GAC antibody 81486-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Am J Cancer Res

IGF2BP3-mediated regulation of GLS and GLUD1 gene expression promotes treg-induced immune escape in human cervical cancer

Authors - Tiantian Zhou
View Article
humanIHC

Front Endocrinol (Lausanne)

Hypoxia-inducible factor-2α promotes fibrosis in non-alcoholic fatty liver disease by enhancing glutamine catabolism and inhibiting yes-associated protein phosphorylation in hepatic stellate cells

Authors - Ranran Yan
View Article