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Phospho-SMAD1 (Ser214) Recombinant antibody

Phospho-SMAD1 (Ser214) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3K8

Cat no : 81433-1-RR

Synonyms

SMAD1, JV4-1, JV41, hSMAD1, BSP-1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of A431 using 81433-1-RR

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of HepG2 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of HepG2 using 81433-1-RR

    Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of NIH/3T3 using 81433-1-RR

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Tested Applications

Positive WB detected inUV treated A431 cells, UV treated NIH/3T3 cells, Calyculin A treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81433-1-RR targets Phospho-SMAD1 (Ser214) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name SMAD family member 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser214) antibody 81433-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols