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  • Featured Product
  • KD/KO Validated

Phospho-MNK1 (Thr250/255) Recombinant antibody

Phospho-MNK1 (Thr250/255) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4P9

Cat no : 81398-1-RR

Synonyms

MKNK1, MNK1, Phospho-MKNK1 (Thr250/255), Phospho-MNK1 (Thr250/255)

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using Phospho-MNK1 (Thr250/255) Recombinant antibody (81398-1-RR)

    WB analysis of HEK-293T using 81398-1-RR

    Non-treated HEK-293T cells, phosphatase inhibitor and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81398-1-RR (Phospho-MNK1 (Thr250/255) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-MNK1 (Thr250/255) Recombinant antibody (81398-1-RR)

    WB analysis of HEK-293 using 81398-1-RR

    WB result of Phospho-MNK1 (Thr250/255) antibody (81398-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-MNK1 (Thr250/255) transfected HEK-293 cells.

Tested Applications

Positive WB detected inHEK-293T cells, HEK-293 cells, λ phosphatase treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81398-1-RR targets Phospho-MNK1 (Thr250/255) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name MAP kinase interacting serine/threonine kinase 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC002755
Gene Symbol MNK1
Gene ID (NCBI) 8569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MNK1/MKNK1 (MAPK Interacting Serine/Threonine Kinase 1) is a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. MNK1 regulates diverse biologic processes including translation, cell proliferation, and differentiation through phosphorylation of different substrates(PMID: 29045529). Through phosphorylation MNK1 regulates the function of eukaryotic translation initiation factor 4E (eIF4E), a key player in translational control, whose expression is mostly upregulated in cancer patients.

Protocols

Product Specific Protocols
WB protocol for Phospho-MNK1 (Thr250/255) antibody 81398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols