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GRB10 Recombinant antibody

GRB10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D13

Cat no : 81225-1-RR

Synonyms

KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRB10 Recombinant antibody (81225-1-RR)

    WB analysis using 81225-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GRB10 Recombinant antibody (81225-1-RR)

    IF Staining of LNCaP using 81225-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81225-1-RR

    Affinity and Kinetic Characterization of 81225-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81225-1-RR against Human GRB10 were performed. The affinity constant is 0.195 nM.

Tested Applications

Positive WB detected inPC-3 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81225-1-RR targets GRB10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRB10 fusion protein Ag20355
Full Name growth factor receptor-bound protein 10
Calculated Molecular Weight536aa,61 kDa; 594aa,67 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GRB10 antibody 81225-1-RRDownload protocol
IF protocol for GRB10 antibody 81225-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols