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  • Featured Product
  • KD/KO Validated

DHFR Recombinant antibody

DHFR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7P5

Cat no : 81021-1-RR

Synonyms

EC:1.5.1.3, DHFRP1, 7P5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHFR Recombinant antibody (81021-1-RR)

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DHFR Recombinant antibody (81021-1-RR)

    WB analysis of HeLa using 81021-1-RR

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DHFR Recombinant antibody (81021-1-RR)

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Recombinant antibody (81021-1-RR)

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using DHFR Recombinant antibody (81021-1-RR)

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HEK-293 cells, MOLT-4 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81021-1-RR targets DHFR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DHFR fusion protein Ag7331
Full Name dihydrofolate reductase
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

Protocols

Product Specific Protocols
WB protocol for DHFR antibody 81021-1-RRDownload protocol
IHC protocol for DHFR antibody 81021-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols