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YTHDF1 Recombinant antibody

YTHDF1 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241445B10

Cat no : 80876-2-RR

Synonyms

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDF1 Recombinant antibody (80876-2-RR)

    WB analysis using 80876-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using YTHDF1 Recombinant antibody (80876-2-RR)

    IF Staining of mouse brain using 80876-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using YTHDF1 Recombinant antibody (80876-2-RR)

    FC experiment of HEK-293 using 80876-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 80876-2-RR

    Affinity and Kinetic Characterization of 80876-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 80876-2-RR is YTHDF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YTHDF1 fusion protein Ag11509
Full Name YTH domain family, member 1
Calculated Molecular Weight 559 aa, 61 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC050284
Gene Symbol YTHDF1
Gene ID (NCBI) 54915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for YTHDF1 antibody 80876-2-RRDownload protocol
IF protocol for YTHDF1 antibody 80876-2-RRDownload protocol
FC protocol for YTHDF1 antibody 80876-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols