Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 80821-2-RR
IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug.
IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug.
IF Staining of HeLa using 80821-2-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of A431 using 80821-2-RR
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
FC experiment of HeLa using 80821-2-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Affinity and Kinetic Characterization of 80821-2-RR
Biolayer interferometry (BLl) kinetic assays of 80821-2-RR against Human p38 MAPK were performed. The affinity constant is 0.435 nM.
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PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
Various lysates were subjected to SDS PAGE followed by western blot with 80821-2-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP Figures
IP experiment of HEK-293 using 80821-2-RR
IP result of anti-p38 MAPK (IP:80821-2-RR, 4ug; Detection:80821-2-RR 1:2000) with HEK-293 cells lysate 1575 ug.
IF/ICC Figures
IF Staining of HeLa using 80821-2-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p38 MAPK antibody (80821-2-RR, Clone: 230263D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC (INTRA) Figures
FC experiment of A431 using 80821-2-RR
1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
FC experiment of HeLa using 80821-2-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-2-RR, Clone:230263D11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
AFFINITY Figures
Affinity and Kinetic Characterization of 80821-2-RR
Biolayer interferometry (BLl) kinetic assays of 80821-2-RR against Human p38 MAPK were performed. The affinity constant is 0.435 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
p38 MAPK Recombinant antibody
Catalog Number
80821-2-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:125-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
