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Phospho-TSC2 (Thr1462) Recombinant antibody

Phospho-TSC2 (Thr1462) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1L20

Cat no : 80698-1-RR

Synonyms

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-TSC2 (Thr1462) Recombinant antibody (80698-1-RR)

    WB analysis of HEK-293 using 80698-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80698-1-RR (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control.

Tested Applications

Positive WB detected inCalyculin A treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80698-1-RR targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name tuberous sclerosis 2
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)

Protocols

Product Specific Protocols
WB protocol for Phospho-TSC2 (Thr1462) antibody 80698-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols