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Connexin 43 Recombinant antibody

Connexin 43 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3O6

Cat no : 80543-1-RR

Synonyms

GJA1, Connexin-43, 3O6, Gap junction alpha-1 protein, Gap junction alpha 1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Connexin 43 Recombinant antibody (80543-1-RR)

    WB analysis using 80543-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IHC staining of mouse heart using 80543-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IHC staining of mouse heart using 80543-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of mouse heart using 80543-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of mouse heart using 80543-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Connexin 43 Recombinant antibody (80543-1-RR)

    IF Staining of HeLa using 80543-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80543-1-RR

    Affinity and Kinetic Characterization of 80543-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80543-1-RR against Human Connexin 43 were performed. The affinity constant is 5.19 nM.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, pig brain tissue, rat cerebellum tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80543-1-RR targets Connexin 43 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Connexin 43 fusion protein Ag25533
Full Name gap junction protein, alpha 1, 43kDa
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC026329
Gene Symbol Connexin-43
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Protocols

Product Specific Protocols
WB protocol for Connexin 43 antibody 80543-1-RRDownload protocol
IHC protocol for Connexin 43 antibody 80543-1-RRDownload protocol
IF protocol for Connexin 43 antibody 80543-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols