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P62,SQSTM1 Recombinant antibody
P62,SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
1D17
Cat no : 80294-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
80294-1-RR targets P62,SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | P62,SQSTM1 fusion protein Ag13131 |
| Full Name | sequestosome 1 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 62 kDa |
| GenBank Accession Number | BC017222 |
| Gene Symbol | P62/SQSTM1 |
| Gene ID (NCBI) | 8878 |
| RRID | AB_2918883 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for P62,SQSTM1 antibody 80294-1-RR | Download protocol |
| IHC protocol for P62,SQSTM1 antibody 80294-1-RR | Download protocol |
| IF protocol for P62,SQSTM1 antibody 80294-1-RR | Download protocol |
| IP protocol for P62,SQSTM1 antibody 80294-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Inhibition of NNMT enhances drug sensitivity in lung cancer cells through mediation of autophagy | ||
Oncogene Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis |
