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  • Western Blot (WB) analysis of various lysates using Nanog Recombinant antibody (80210-13-RR)

    WB analysis using 80210-13-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Nanog Recombinant antibody (80210-13-RR)

    WB analysis using 80210-13-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using Nanog Recombinant antibody (80210-13-RR)

    IF Staining of NCCIT using 80210-13-RR

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80210-13-RR

    Affinity and Kinetic Characterization of 80210-13-RR

    Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

Nanog Recombinant antibody

Nanog Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80210-13-RR
Clone No.250067F10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

hNanog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, rat brain tissue, C2C12 cells
Positive IF/ICC detected inNCCIT cells

This antibody is not recommended for WB detection of human samples.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80210-13-RR targets Nanog in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Nanog fusion protein Ag5645 Predict reactive species
Full Name Nanog homeobox
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC160187
Gene Symbol Nanog
Gene ID (NCBI) 79923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H9S0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Protocols

Product Specific Protocols
WB protocol for Nanog antibody 80210-13-RRDownload protocol
IF protocol for Nanog antibody 80210-13-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols