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NeutraKine® GM-CSF Monoclonal antibody, PBS Only (Capture)

NeutraKine® GM-CSF Monoclonal Antibody for Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10E7

Cat no : 69003-1-PBS

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, NeutraKine® GM-CSF, Sargramostim

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1

    Sandwich ELISA standard curve of MP50067-1, GM-CSF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 69003-1-PBS. Detection antibody: 68702-1-PBS. Standard: Eg0189. Range: 7.8-500 pg/mL.

  • Neutralization experiment of NeutraKine® GM-CSF using NeutraKine® GM-CSF Monoclonal antibody (69003-1-Ig)

    Neutralization experiment of NeutraKine® GM-CSF using 69003-1-Ig (same clone as 69003-1-PBS)

    Recombinant human GM-CSF (Cat.NO. HZ-1002) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human GM-CSF (0.2 ng/mL HZ-1002) is neutralized by mouse anti-human GM-CSF monoclonal antibody 69003-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1.5-4 μg/mL This data was developed using the same antibody clone with 69003-1-PBS in a different storage buffer formulation.

Product Information

69003-1-PBS targets NeutraKine® GM-CSF as part of a matched antibody pair:

MP50067-1: 69003-1-PBS capture and 68702-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine GM-CSF protein HZ-1002
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

This antibody can be used to neutralize the bioactivity of GM-CSF.