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HJURP Monoclonal antibody

HJURP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F8C5

Cat no : 68995-1-Ig

Synonyms

Up regulated in lung cancer 9, hFLEG1, FLEG1, Fetal liver-expressing gene 1 protein, FAKTS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HJURP Monoclonal antibody (68995-1-Ig)

    WB analysis using 68995-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPANC-1 cells, SW 1990 cells, Saos2 cells, U2OS cells, A549 cells, HeLa cells, MDA-MB-468 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68995-1-Ig targets HJURP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HJURP fusion protein Ag34571
Full Name Holliday junction recognition protein
Calculated Molecular Weight 86 kDa
Observed Molecular Weight85-90 kDa
GenBank Accession NumberBC001940
Gene Symbol HJURP
Gene ID (NCBI) 55355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

Protocols

Product Specific Protocols
WB protocol for HJURP antibody 68995-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols