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MLXIP Monoclonal antibody

MLXIP Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E11A4

Cat no : 68977-1-Ig

Synonyms

MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36, BHLHE36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLXIP Monoclonal antibody (68977-1-Ig)

    WB analysis using 68977-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68977-1-Ig (MLXIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inACHN cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, Mo7e cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68977-1-Ig targets MLXIP in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MLXIP fusion protein Ag4519
Full Name MLX interacting protein
Calculated Molecular Weight 919 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039704
Gene Symbol MLXIP
Gene ID (NCBI) 22877
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Protocols

Product Specific Protocols
WB protocol for MLXIP antibody 68977-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols