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ICT1 Monoclonal antibody

ICT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F11E6

Cat no : 68898-3-Ig

Synonyms

Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1, 39S ribosomal protein L58, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis using 68898-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis using 68898-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW480 cells using ICT1 Monoclonal antibody (68898-3-Ig)

    WB analysis of SW480 using 68898-3-Ig

    SW480 cells were subjected to SDS PAGE followed by western blot with 68898-3-Ig (ICT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHCT 116 cells, Calu-3 cells, SW480 cells, LNCaP cells, HeLa cells, HEK-293 cells, SK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68898-3-Ig targets ICT1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ICT1 fusion protein Ag33109
Full Name immature colon carcinoma transcript 1
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberBC015335
Gene Symbol ICT1
Gene ID (NCBI) 3396
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells.

Protocols

Product Specific Protocols
WB protocol for ICT1 antibody 68898-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols