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  • Western Blot (WB) analysis of various lysates using ADCK4 Monoclonal antibody (68877-1-Ig)

    WB analysis using 68877-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68877-1-Ig (ADCK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ADCK4 Monoclonal antibody

ADCK4 Monoclonal Antibody for WB, ELISA
Cat No. 68877-1-Ig
Clone No.1B7E3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.-.-, COQ8B, Coenzyme Q protein 8B, Atypical kinase COQ8B, mitochondrial, AarF domain-containing protein kinase 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68877-1-Ig targets ADCK4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADCK4 fusion protein Ag13188 Predict reactive species
Full Name aarF domain containing kinase 4
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC013114
Gene Symbol ADCK4
Gene ID (NCBI) 79934
RRIDAB_3670444
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96D53
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADCK4 (AarF domain-containing protein kinase 4), also known as COQ8B (Coenzyme Q protein 8B), is an atypical kinase involved in the biosynthesis of coenzyme Q, which is also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PMID: 24270420). Its substrate specificity is unclear: does not show any protein kinase activity. It probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway (PMID: 24270420).

Protocols

Product Specific Protocols
WB protocol for ADCK4 antibody 68877-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols