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ZC3H12D Monoclonal antibody

ZC3H12D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 68847-1-Ig

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZC3H12D Monoclonal antibody (68847-1-Ig)

    WB analysis using 68847-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68847-1-Ig (ZC3H12D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inKarpas-422 cells, MJ cells, Raji cells, Ramos cells, Daudi cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68847-1-Ig targets ZC3H12D in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ZC3H12D fusion protein Ag21770
Full Name zinc finger CCCH-type containing 12D
Calculated Molecular Weight 527 aa, 58 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC157832
Gene Symbol ZC3H12D
Gene ID (NCBI) 340152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

Protocols

Product Specific Protocols
WB protocol for ZC3H12D antibody 68847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols