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CPA1 Monoclonal antibody

CPA1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H2E8

Cat no : 68826-1-Ig

Synonyms

Carboxypeptidase A1, EC:3.4.17.1, CPA, 2H2E8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CPA1 Monoclonal antibody (68826-1-Ig)

    WB analysis using 68826-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig pancreas tissue, rat pancreas tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68826-1-Ig targets CPA1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CPA1 fusion protein Ag8579
Full Name carboxypeptidase A1 (pancreatic)
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC005279
Gene Symbol Carboxypeptidase A1
Gene ID (NCBI) 1357
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

Protocols

Product Specific Protocols
WB protocol for CPA1 antibody 68826-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols