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CHMP4B Monoclonal antibody

CHMP4B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A3F5

Cat no : 68811-2-Ig

Synonyms

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, dJ553F4.4, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Monoclonal antibody (68811-2-Ig)

    WB analysis using 68811-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68811-2-Ig (CHMP4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CHMP4B Monoclonal antibody (68811-2-Ig)

    IF Staining of HCT 116 using 68811-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using CHMP4B antibody (68811-2-Ig, Clone: 2A3F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68811-2-Ig targets CHMP4B in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHMP4B fusion protein Ag33487
Full Name chromatin modifying protein 4B
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Protocols

Product Specific Protocols
WB protocol for CHMP4B antibody 68811-2-IgDownload protocol
IF protocol for CHMP4B antibody 68811-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols