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ALPL Monoclonal antibody

ALPL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E3C1

Cat no : 68719-1-Ig

Synonyms

ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALPL Monoclonal antibody (68719-1-Ig)

    WB analysis using 68719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68719-1-Ig (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inpig kidney tissue, rabbit kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68719-1-Ig targets ALPL in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ALPL fusion protein Ag33678
Full Name alkaline phosphatase, liver/bone/kidney
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

Protocols

Product Specific Protocols
WB protocol for ALPL antibody 68719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols