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KIAA0319L Monoclonal antibody

KIAA0319L Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E11D9

Cat no : 68717-1-Ig

Synonyms

KIAA0319 like, KIAA0319L, KIAA1837, PKD1 like, PP791, RP4 765A10.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA0319L Monoclonal antibody (68717-1-Ig)

    WB analysis using 68717-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68717-1-Ig (KIAA0319L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68717-1-Ig targets KIAA0319L in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KIAA0319L fusion protein Ag15263
Full Name KIAA0319-like
Calculated Molecular Weight 1049 aa, 116 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC031672
Gene Symbol KIAA0319L
Gene ID (NCBI) 79932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

Protocols

Product Specific Protocols
WB protocol for KIAA0319L antibody 68717-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols