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TNNT1 Monoclonal antibody

TNNT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1D5

Cat no : 68631-1-Ig

Synonyms

TNTS, TNT, STNT, ANM

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNNT1 Monoclonal antibody (68631-1-Ig)

    WB analysis using 68631-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68631-1-Ig (TNNT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TNNT1 Monoclonal antibody (68631-1-Ig)

    IF Staining of hTERT-RPE1 using 68631-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TNNT1 antibody (68631-1-Ig, Clone: 1B1D5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68631-1-Ig targets TNNT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TNNT1 fusion protein Ag8919
Full Name troponin T type 1 (skeletal, slow)
Calculated Molecular Weight252aa,27 kDa; 192aa,21 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC022086
Gene Symbol TNNT1
Gene ID (NCBI) 7138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TNNT1 antibody 68631-1-IgDownload protocol
IF protocol for TNNT1 antibody 68631-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols