Filter: AllWBIHCCytometric bead array

WB analysis using 68627-1-Ig (same clone as 68627-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 68627-1-Ig (same clone as 68627-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
WB analysis of rat colon using 68627-1-Ig (same clone as 68627-1-PBS)
rat colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
WB analysis of mouse colon using 68627-1-Ig (same clone as 68627-1-PBS)
mouse colon tissue were subjected to SDS PAGE followed by western blot with 68627-1-Ig (KRT8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 68627-1-Ig (same clone as 68627-1-PBS)
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 68627-1-Ig (same clone as 68627-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-1-Ig (Cytokeratin 8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68627-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51080-1
Cytometric bead array standard curve of MP51080-1, Cytokeratin 8 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68627-1-PBS. Detection antibody: 68627-2-PBS. Standard:Ag0488. Range: 0.098-100 ng/mL.

Cytokeratin 8 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 8 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 68627-1-PBS

Clone No.1F2H5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

KRT8, Cytokeratin,Keratin, CK-8, CK8, CK 8

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

68627-1-PBS targets Cytokeratin 8 as part of a matched antibody pair:

MP51080-1: 68627-1-PBS capture and 68627-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Cytokeratin 8 fusion protein Ag0488 Predict reactive species
Full Name	keratin 8
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	50-52 kDa
GenBank Accession Number	BC000654
Gene Symbol	Cytokeratin 8
Gene ID (NCBI)	3856
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P05787
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.