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RSAD2 Monoclonal antibody

RSAD2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C5D4

Cat no : 68604-1-Ig

Synonyms

2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using RSAD2 Monoclonal antibody (68604-1-Ig)

    WB analysis of pig liver using 68604-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68604-1-Ig (RSAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RSAD2 Monoclonal antibody (68604-1-Ig)

    WB analysis using 68604-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68604-1-Ig (RSAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inpig liver tissue, mouse liver tissue, mouse brain tissue, pig spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68604-1-Ig targets RSAD2 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RSAD2 fusion protein Ag27733
Full Name radical S-adenosyl methionine domain containing 2
Calculated Molecular Weight 361 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC017969
Gene Symbol RSAD2
Gene ID (NCBI) 91543
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.

Protocols

Product Specific Protocols
WB protocol for RSAD2 antibody 68604-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols