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RAB38 Monoclonal antibody

RAB38 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A10C5

Cat no : 68603-1-Ig

Synonyms

Melanoma antigen NY MEL 1, NY MEL 1, RAB38, Ras related protein Rab 38, rrGTPbp

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB38 Monoclonal antibody (68603-1-Ig)

    WB analysis using 68603-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68603-1-Ig (RAB38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Mo7e cells using RAB38 Monoclonal antibody (68603-1-Ig)

    WB analysis of Mo7e using 68603-1-Ig

    Mo7e cells were subjected to SDS PAGE followed by western blot with 68603-1-Ig (RAB38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-10A cells, Mo7e cells, HaCaT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68603-1-Ig targets RAB38 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAB38 fusion protein Ag26440
Full Name RAB38, member RAS oncogene family
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC015808
Gene Symbol RAB38
Gene ID (NCBI) 23682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The RAB family of small GTPases is part of the Ras protein superfamily and is primarily responsible for regulating intracellular vesicle transport and migration. RAB38 is a recently identified member of the RAB family of proteins and its expression is tissue-specific. RAB38 is predominantly expressed in melanocytes and melanoma tissues, but not in other normal tissues, serving as a melanocyte differentiation antigen. RAB38 has also been reported to be upregulated in glioblastoma, and high levels of its expression are associated with a poor prognosis and enhanced cell migration.

Protocols

Product Specific Protocols
WB protocol for RAB38 antibody 68603-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols