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NGRN Monoclonal antibody

NGRN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C2C9

Cat no : 68593-1-Ig

Synonyms

DSC92, FI58G, Neugrin, NGRN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NGRN Monoclonal antibody (68593-1-Ig)

    WB analysis using 68593-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68593-1-Ig (NGRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68593-1-Ig targets NGRN in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NGRN fusion protein Ag6790
Full Name neugrin, neurite outgrowth associated
Calculated Molecular Weight 32 kDa
Observed Molecular Weight30-32 kDa
GenBank Accession NumberBC001682
Gene Symbol NGRN
Gene ID (NCBI) 51335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.

Protocols

Product Specific Protocols
WB protocol for NGRN antibody 68593-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols