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IQGAP1 Monoclonal antibody

IQGAP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E11A2

Cat no : 68588-1-Ig

Synonyms

Ras GTPase-activating-like protein IQGAP1, p195, KIAA0051, HUMORFA01, 2E11A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IQGAP1 Monoclonal antibody (68588-1-Ig)

    WB analysis using 68588-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68588-1-Ig (IQGAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IQGAP1 Monoclonal antibody (68588-1-Ig)

    IHC staining of human skin cancer using 68588-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68588-1-Ig (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, A549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68588-1-Ig targets IQGAP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IQGAP1 fusion protein Ag19321
Full Name IQ motif containing GTPase activating protein 1
Calculated Molecular Weight 189 kDa
Observed Molecular Weight 190-195 kDa
GenBank Accession NumberBC151834
Gene Symbol IQGAP1
Gene ID (NCBI) 8826
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Protocols

Product Specific Protocols
WB protocol for IQGAP1 antibody 68588-1-IgDownload protocol
IHC protocol for IQGAP1 antibody 68588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols