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THOP1 Monoclonal antibody

THOP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse , Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B11A11

Cat no : 68587-1-Ig

Synonyms

Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOP1 Monoclonal antibody (68587-1-Ig)

    WB analysis using 68587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68587-1-Ig (THOP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using THOP1 Monoclonal antibody (68587-1-Ig)

    WB analysis of HeLa using 68587-1-Ig

    WB result of THOP1 antibody (68587-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THOP1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using THOP1 Monoclonal antibody (68587-1-Ig)

    WB analysis using 68587-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68587-1-Ig (THOP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68587-1-Ig targets THOP1 in WB, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen THOP1 fusion protein Ag2268
Full Name thimet oligopeptidase 1
Calculated Molecular Weight 689 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC000135
Gene Symbol THOP1
Gene ID (NCBI) 7064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

Protocols

Product Specific Protocols
WB protocol for THOP1 antibody 68587-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols