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BCAT2 Monoclonal antibody

BCAT2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A5

Cat no : 68586-1-Ig

Synonyms

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAT2 Monoclonal antibody (68586-1-Ig)

    WB analysis using 68586-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using BCAT2 Monoclonal antibody (68586-1-Ig)

    IF Staining of A431 using 68586-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inA431 cells, HCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, mouse testis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:565-1:2260
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68586-1-Ig targets BCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BCAT2 fusion protein Ag9595
Full Name branched chain aminotransferase 2, mitochondrial
Calculated Molecular Weight 44 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC001900
Gene Symbol BCAT2
Gene ID (NCBI) 587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

Protocols

Product Specific Protocols
WB protocol for BCAT2 antibody 68586-1-IgDownload protocol
IF protocol for BCAT2 antibody 68586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols