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CYP46A1 Monoclonal antibody

CYP46A1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E10D3

Cat no : 68576-1-Ig

Synonyms

CH24H, Cholesterol 24 hydroxylase, CP46, CYP46, CYP46A1, Cytochrome P450 46A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP46A1 Monoclonal antibody (68576-1-Ig)

    WB analysis using 68576-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68576-1-Ig (CYP46A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP46A1 Monoclonal antibody (68576-1-Ig)

    IHC staining of mouse brain using 68576-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CYP46A1 Monoclonal antibody (68576-1-Ig)

    IHC staining of mouse brain using 68576-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68576-1-Ig (CYP46A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68576-1-Ig targets CYP46A1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYP46A1 fusion protein Ag16786
Full Name cytochrome P450, family 46, subfamily A, polypeptide 1
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC022539
Gene Symbol CYP46A1
Gene ID (NCBI) 10858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

Protocols

Product Specific Protocols
WB protocol for CYP46A1 antibody 68576-1-IgDownload protocol
IHC protocol for CYP46A1 antibody 68576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols