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MAP2K3 Monoclonal antibody

MAP2K3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H3E10

Cat no : 68574-1-Ig

Synonyms

MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2K3 Monoclonal antibody (68574-1-Ig)

    WB analysis using 68574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68574-1-Ig (MAP2K3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MAP2K3 Monoclonal antibody (68574-1-Ig)

    WB analysis of A431 using 68574-1-Ig

    WB result of MAP2K3 antibody (68574-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected A431 cells.

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells, MCF-7 cells, HeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68574-1-Ig targets MAP2K3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAP2K3 fusion protein Ag4861
Full Name mitogen-activated protein kinase kinase 3
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC032478
Gene Symbol MAP2K3
Gene ID (NCBI) 5606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAP2K3 antibody 68574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols