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LEO1 Monoclonal antibody

LEO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D7A2

Cat no : 68568-1-Ig

Synonyms

LEO1, RDL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LEO1 Monoclonal antibody (68568-1-Ig)

    WB analysis using 68568-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68568-1-Ig (LEO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inHepG2 cells, HT-29 cells, A549 cells, HEK-293 cells, LNCaP cells, MCF-7 cells, Hela cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68568-1-Ig targets LEO1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LEO1 fusion protein Ag33577
Full Name Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated Molecular Weight 666 aa, 75 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC018147
Gene Symbol LEO1
Gene ID (NCBI) 123169
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Protocols

Product Specific Protocols
WB protocol for LEO1 antibody 68568-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols