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RPAIN Monoclonal antibody

RPAIN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8E3

Cat no : 68546-1-Ig

Synonyms

HRIP, RIP, RPA interacting protein, RPAIN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis using 68546-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis using 68546-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using RPAIN Monoclonal antibody (68546-1-Ig)

    WB analysis of A2780 using 68546-1-Ig

    A2780 cells were subjected to SDS PAGE followed by western blot with 68546-1-Ig (RPAIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, A2780 cells, LNCap cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68546-1-Ig targets RPAIN in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPAIN fusion protein Ag7844
Full Name RPA interacting protein
Calculated Molecular Weight 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC004451
Gene Symbol RPAIN
Gene ID (NCBI) 84268
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. RPAIN was originally identified as a nuclear transporter of RPA in Xenopus (PMID: 23010595). The human RIPα gene encodes several splice isoforms, of which hRIPα and hRIPβ are the major translation products in vivo.hRIPα transports RPA into the nucleus by shuttling between the cytoplasm and nucleus while hRIPβ is localized in promyelocytic leukemia (PML) nuclear bodies.

Protocols

Product Specific Protocols
WB protocol for RPAIN antibody 68546-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols