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MYST2 Monoclonal antibody

MYST2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1B1

Cat no : 68525-1-Ig

Synonyms

HBO1, HBOA, KAT7, MYST 2, MYST2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYST2 Monoclonal antibody (68525-1-Ig)

    WB analysis using 68525-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68525-1-Ig (MYST2 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68525-1-Ig targets MYST2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MYST2 fusion protein Ag5166
Full Name MYST histone acetyltransferase 2
Calculated Molecular Weight 611 aa, 71 kDa
Observed Molecular Weight83 kDa
GenBank Accession NumberBC032640
Gene Symbol MYST2
Gene ID (NCBI) 11143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

Protocols

Product Specific Protocols
WB protocol for MYST2 antibody 68525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols