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POLR2F Monoclonal antibody

POLR2F Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D4D12

Cat no : 68511-1-Ig

Synonyms

HRBP14.4, POLR2F, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPB6 homolog, RPC15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POLR2F Monoclonal antibody (68511-1-Ig)

    WB analysis using 68511-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLR2F Monoclonal antibody (68511-1-Ig)

    WB analysis using 68511-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68511-1-Ig (POLR2F antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, MCF-7 cells, A549 cells, HeLa cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68511-1-Ig targets POLR2F in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen POLR2F fusion protein Ag7472
Full Name polymerase (RNA) II (DNA directed) polypeptide F
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC003582
Gene Symbol POLR2F
Gene ID (NCBI) 5435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR2F antibody 68511-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols