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CDCA8 Monoclonal antibody

CDCA8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C9

Cat no : 68510-1-Ig

Synonyms

Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin, BOR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA8 Monoclonal antibody (68510-1-Ig)

    WB analysis using 68510-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CDCA8 Monoclonal antibody (68510-1-Ig)

    IF Staining of A431 using 68510-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CDCA8 Monoclonal antibody (68510-1-Ig)

    IF Staining of A549 using 68510-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inMDA-MB-468 cells, A431 cells, HEK-293 cell, K-562 cells, Ramos cells, Daudi cells
Positive IF/ICC detected inA549 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68510-1-Ig targets CDCA8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDCA8 fusion protein Ag3136
Full Name cell division cycle associated 8
Calculated Molecular Weight 280 aa, 31 kDa
Observed Molecular Weight 31-38 kDa
GenBank Accession NumberBC016944
Gene Symbol CDCA8
Gene ID (NCBI) 55143
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

Protocols

Product Specific Protocols
WB protocol for CDCA8 antibody 68510-1-IgDownload protocol
IF protocol for CDCA8 antibody 68510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols