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TEX264 Monoclonal antibody

TEX264 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D12G1

Cat no : 68507-1-Ig

Synonyms

DKFZp451H0417, FLJ13935, SIG11, testis expressed 264, TEX264, ZSIG11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TEX264 Monoclonal antibody (68507-1-Ig)

    WB analysis using 68507-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68507-1-Ig (TEX264 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68507-1-Ig targets TEX264 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TEX264 fusion protein Ag33710
Full Name testis expressed 264
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC008742
Gene Symbol TEX264
Gene ID (NCBI) 51368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Protocols

Product Specific Protocols
WB protocol for TEX264 antibody 68507-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols