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ATP6V0D1 Monoclonal antibody

ATP6V0D1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G7A12

Cat no : 68506-1-Ig

Synonyms

32 kDa accessory protein, ATP6D, ATP6DV, ATP6V0D1, P39, V ATPase AC39 subunit, V ATPase subunit d 1, VATX, VMA6, VPATPD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    WB analysis using 68506-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IP experiment of HeLa using 68506-1-Ig

    IP result of anti-ATP6V0D1 (IP:68506-1-Ig, 4ug; Detection:68506-1-Ig 1:3000) with HeLa cells lysate 1520 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IHC staining of mouse kidney using 68506-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IHC staining of mouse kidney using 68506-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68506-1-Ig (ATP6V0D1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATP6V0D1 Monoclonal antibody (68506-1-Ig)

    IF Staining of HeLa using 68506-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATP6V0D1 antibody (68506-1-Ig, Clone: 1G7A12 ) at dilution of 1:2400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, zebrafish tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:1200-1:4800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68506-1-Ig targets ATP6V0D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken, Zebrafish
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP6V0D1 fusion protein Ag13012
Full Name ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight37-41 kDa
GenBank Accession NumberBC008861
Gene Symbol ATP6V0D1
Gene ID (NCBI) 9114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V0D1(V-type proton ATPase subunit d 1) is also named as ATP6D, VPATPD and belongs to the V-ATPase V0D/AC39 subunit family.It is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Protocols

Product Specific Protocols
WB protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IHC protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IF protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
IP protocol for ATP6V0D1 antibody 68506-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols