Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 68500-1-Ig
WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 68500-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68500-1-Ig targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).
Various lysates were subjected to SDS PAGE followed by western blot with 68500-1-Ig (PSPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 68500-1-Ig
WB result of PSPH antibody (68500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected A375 cells.
IHC Figures
IHC staining of mouse spleen using 68500-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 68500-1-Ig (PSPH antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HL-60 using 68500-1-Ig
IP result of anti-PSPH (IP:68500-1-Ig, 4ug; Detection:68500-1-Ig 1:5000) with HL-60 cells lysate 2440 ug.
IF/ICC Figures
IF Staining of HepG2 using 68500-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (68500-1-Ig, Clone: 1B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
KD/KO VALIDATED
PSPH Monoclonal antibody
Catalog Number
68500-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:2000-1:8000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
