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STT3B Monoclonal antibody

STT3B Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B7A8

Cat no : 68496-1-Ig

Synonyms

STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STT3B Monoclonal antibody (68496-1-Ig)

    WB analysis using 68496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using STT3B Monoclonal antibody (68496-1-Ig)

    WB analysis using 68496-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Flow cytometry (FC) experiment of A431 cells using STT3B Monoclonal antibody (68496-1-Ig)

    FC experiment of A431 using 68496-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.8 ug STT3B Monoclonal antibody (68496-1-Ig, Clone:3B7A8) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68496-1-Ig targets STT3B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STT3B fusion protein Ag30866
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC015880
Gene Symbol STT3B
Gene ID (NCBI) 201595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity

Protocols

Product Specific Protocols
WB protocol for STT3B antibody 68496-1-IgDownload protocol
FC protocol for STT3B antibody 68496-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols