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NUDT10 Monoclonal antibody

NUDT10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A8D5

Cat no : 68478-1-Ig

Synonyms

APS2, DIPP 3 alpha, DIPP3 alpha, DIPP3a, hAps2, hDIPP3alpha, Nudix motif 10, NUDT10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT10 Monoclonal antibody (68478-1-Ig)

    WB analysis using 68478-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68478-1-Ig (NUDT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUDT10 Monoclonal antibody (68478-1-Ig)

    WB analysis using 68478-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68478-1-Ig (NUDT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, rat brain tissue, HEK-293 cells, rat testis tissue, mouse testis tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68478-1-Ig targets NUDT10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NUDT10 fusion protein Ag11878
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 10
Calculated Molecular Weight 164 aa, 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC050700
Gene Symbol NUDT10
Gene ID (NCBI) 170685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228).

Protocols

Product Specific Protocols
WB protocol for NUDT10 antibody 68478-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols