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Tested Applications
| Positive WB detected in | HCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68464-1-Ig targets TEAD3 in WB, ELISA applications and shows reactivity with Human, pig samples.
| Tested Reactivity | Human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TEAD3 fusion protein Ag4407 Predict reactive species |
| Full Name | TEA domain family member 3 |
| Calculated Molecular Weight | 435 aa, 49 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC027877 |
| Gene Symbol | TEAD3 |
| Gene ID (NCBI) | 7005 |
| RRID | AB_3085175 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99594 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEAD3 antibody 68464-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
