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ATXN10 Monoclonal antibody

ATXN10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4E4

Cat no : 68455-1-Ig

Synonyms

ataxin 10, ATXN10, Brain protein E46 homolog, E46L, FLJ37990, SCA10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATXN10 Monoclonal antibody (68455-1-Ig)

    WB analysis using 68455-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68455-1-Ig (ATXN10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68455-1-Ig targets ATXN10 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATXN10 fusion protein Ag8254
Full Name ataxin 10
Calculated Molecular Weight 53 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC007508
Gene Symbol ATXN10
Gene ID (NCBI) 25814
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

Protocols

Product Specific Protocols
WB protocol for ATXN10 antibody 68455-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols