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FBP1 Monoclonal antibody

FBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G4E4

Cat no : 68446-1-Ig

Synonyms

FBP, FBP1, FBPase 1, fructose 1,6 bisphosphatase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis using 68446-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of MCF-7 using 68446-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of T-47D using 68446-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    WB analysis of pig liver using 68446-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68446-1-Ig (FBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    IHC staining of mouse kidney using 68446-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FBP1 Monoclonal antibody (68446-1-Ig)

    IHC staining of mouse kidney using 68446-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68446-1-Ig (FBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FBP1 Monoclonal antibody (68446-1-Ig)

    IF Staining of MCF-7 using 68446-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FBP1 antibody (68446-1-Ig, Clone: 2G4E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells, T-47D cells, pig liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:40000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68446-1-Ig targets FBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FBP1 fusion protein Ag3837
Full Name fructose-1,6-bisphosphatase 1
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC012927
Gene Symbol FBP1
Gene ID (NCBI) 2203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBP1 (Fructose-1,6-bisphosphatase 1) is also named as FBP and belongs to the FBPase class 1 family. It catalyzes the hydrolysis of fructose-1,6 bisphosphate to fructose-6-phosphate and inorganic phosphate. This reaction is an important regulatory site of gluconeogenesis. Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) (PMID:12126934).

Protocols

Product Specific Protocols
WB protocol for FBP1 antibody 68446-1-IgDownload protocol
IHC protocol for FBP1 antibody 68446-1-IgDownload protocol
IF protocol for FBP1 antibody 68446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols