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SYAP1 Monoclonal antibody

SYAP1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E12A5

Cat no : 68410-1-Ig

Synonyms

DKFZp686K221, FLJ14495, FLJ44185, PRO3113, SYAP1, Synapse associated protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYAP1 Monoclonal antibody (68410-1-Ig)

    WB analysis using 68410-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68410-1-Ig (SYAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunoprecipitation (IP) experiment of L02 cells using SYAP1 Monoclonal antibody (68410-1-Ig)

    IP experiment of L02 using 68410-1-Ig

    IP result of anti-SYAP1 (IP:68410-1-Ig, 4ug; Detection:68410-1-Ig 1:2000) with L02 cells lysate 3080 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYAP1 Monoclonal antibody (68410-1-Ig)

    IF Staining of HepG2 using 68410-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SYAP1 antibody (68410-1-Ig, Clone: 2E12A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68410-1-Ig targets SYAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SYAP1 fusion protein Ag9626
Full Name synapse associated protein 1, SAP47 homolog (Drosophila)
Calculated Molecular Weight 352 aa, 40 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC014657
Gene Symbol SYAP1
Gene ID (NCBI) 94056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYAP1(Synapse-associated protein 1) is a human homologue of the Drosophila SAP47 (synapse associated protein), which is recognized by a monoclonal antibody that selectively stain synaptic terminals.This protein is a 352 amino acid protein that is ubiquitously expressed in adult tissues. SYAP1 contains one BSD domain which is is an approximately 60 amino acid long protein domain named after the BTF2-like transcription factors, Synapse-associated proteins and DOS2-like proteins in which it is found.

Protocols

Product Specific Protocols
WB protocol for SYAP1 antibody 68410-1-IgDownload protocol
IF protocol for SYAP1 antibody 68410-1-IgDownload protocol
IP protocol for SYAP1 antibody 68410-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols