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STUB1 Monoclonal antibody

STUB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B9

Cat no : 68407-1-Ig

Synonyms

CHIP, E3 ubiquitin-protein ligase CHIP, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, Antigen NY-CO-7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STUB1 Monoclonal antibody (68407-1-Ig)

    WB analysis using 68407-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68407-1-Ig (STUB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using STUB1 Monoclonal antibody (68407-1-Ig)

    IF Staining of MCF-7 using 68407-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using STUB1 antibody (68407-1-Ig, Clone: 2E6B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68407-1-Ig targets STUB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name STIP1 homology and U-box containing protein 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberNM_005861
Gene Symbol STUB1
Gene ID (NCBI) 10273
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 ) This antibody is specific to STUB1.

Protocols

Product Specific Protocols
WB protocol for STUB1 antibody 68407-1-IgDownload protocol
IF protocol for STUB1 antibody 68407-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols