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GATC Monoclonal antibody

GATC Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F9C8

Cat no : 68405-1-Ig

Synonyms

Protein 15E1.2, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial, Glu-AdT subunit C, GatC like protein, EC:6.3.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis of A549 using 68405-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis using 68405-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GATC Monoclonal antibody (68405-1-Ig)

    IF Staining of HEK-293 using 68405-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HEK-293 cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68405-1-Ig targets GATC in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GATC fusion protein Ag19857
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Calculated Molecular Weight 136 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC107145
Gene Symbol GATC
Gene ID (NCBI) 283459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

Protocols

Product Specific Protocols
WB protocol for GATC antibody 68405-1-IgDownload protocol
IF protocol for GATC antibody 68405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols